TopSPEC® PRRSV RT-qPCR KIT (SQV-106)

Intended uses: Detection and differentiation of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type II (North American lineage), characterized by the ORF7 gene, and the highly pathogenic Type II (HP), characterized by the ORF1a gene, associated with disease in pigs using One-step RT-qPCR.

Storage: Store at -20oC

Expiration date: 12 months from the date of manufacture

Manufacturer: ABT Vietnam

PRODUCT CATEGORY

Giới thiệu TopSPEC® PRRSV RT-qPCR KIT

 

The syndrome of respiratory and reproductive disorders in pigs, commonly known as Porcine Reproductive and Respiratory Syndrome (PRRS), is caused by the PRRSV. This is a highly contagious and dangerous infectious disease that spreads rapidly and leads to a significant mortality rate in infected pigs, resulting in substantial losses to the swine farming industry. The disease affects pigs of all ages, with a potential mortality rate of up to 100%. Early detection of the disease within a herd plays a crucial role in mitigating the damages caused by the illness.

TopSPEC® PRRSV RT-qPCR KIT is a diagnostic assay designed to detect the presence of PRRSV, the causative agent of blue ear disease - Porcine Reproductive and Respiratory Syndrome, in pigs. Additionally, the kit enables differentiation between PRRSV Type II (North American lineage), characterized by the ORF7 gene, and the highly pathogenic Type II (HP), characterized by the ORF1a gene, using One-step RT-qPCR.

TopSPEC® PRRSV One-step RT-qPCR KIT

TopSPEC® PRRSV RT-qPCR KIT

Highlights

  • Simple procedure
  • PCR time is optimized for 2 hours
  • High specificity and sensitivity
  • Taqman probe technology

Specification

TargetsPorcine reproductive and respiratory syndrome (PRRSV) Type II (NA) – ORF7 gene và Type II high virulent (HP) – ORF1a gene
Input sampleRNA after extraction
Input sample volume5 µL
Quantitative detectionFAM: ORF7 gene of PRRSV-Type II (NA)
Texas Red: ORF1a gene of PRRSV-Type II-China (HP)
HEX: internal control
Flourescent channelTaqMan probe
PCR time1 hour 45 minutes
SpecificityKit only detects PRRSV-Type II (NA) và PRRSV-Type II-China (HP)
Storage12 months at -20oC
Kit componentsPRRSV One-step RT-qPCR mix, negative control, positive control, internal control (IC), PCR tube

Caution:

  • The detection of the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is most effective in pigs that are impacted during the early stages of PRRSV infection. This includes vulnerable piglets, lethargic pigs, weak nursing piglets, pigs exhibiting signs of anorexia, and recently farrowed sows/sows with signs of fever, anorexia, and clinical symptoms.
  • After sample collection, it is advisable to transport the samples under cold conditions and store them in a freezer until testing is conducted.

 

Extraction kit recommended for use:

Read more: Instructional Video for Qualitative Real-time PCR Kit

Code

Package

SQV-106

50 tests

Manual instruction

Vietnamese version

English version

Catalog

Vietnamese version

English version

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Công ty TNHH giải pháp y sinh ABT

ABT BIOLOGICAL SOLUTIONS COMPANY LIMITED

ABT is a pioneering biotechnology company specializing in advanced diagnostic solutions that support disease prevention and safeguard public health. With a global vision, ABT continuously invests in research and development to enhance product quality and affirm the scientific standing of Vietnam on the international stage.

Contact us

Factory 6.07 and 5.02, Lot L2, Long Hau – Hiep Phuoc Road, Long Hau Industrial Park, Can Giuoc Commune, Tay Ninh Province, Vietnam

No. 1 and No. 3, 13B Street, An Lac Ward, Ho Chi Minh City, Vietnam

0903 307 258
028 2216 0885

oversea@abtvn.com

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