TopSPEC® Isospora suis qPCR KIT (SQV-014)

About TopSPEC® Isospora suis qPCR KIT

Swine coccidiosis is caused by the parasite Isospora suis, affecting pigs of all ages but most commonly observed in neonatal piglets under two weeks old. The disease is prevalent in high-density pig farming areas with poor sanitation conditions. Its characteristic symptoms include enteritis and diarrhea, which may appear as liquid or pasty stool—initially milky white and later turning yellow with a foul odor. Infected piglets may exhibit weakness, dehydration, stunted growth, reduced weight gain, and, in severe cases, death.

TopSPEC® Isospora suis qPCR Kit is a diagnostic kit designed to detect the presence of Isospora suis – ITS1 gene – associated with coccidiosis in pigs using the qPCR technique. The kit utilizes a master mix containing UNG (Uracil-N-Glycosylase), which helps prevent cross-contamination from previous PCR products.

TopSPEC® Isospora suis qPCR Kit

Highlights

• Simple procedure
• The optimized PCR duration is approximately 1.5 hours
• High specificity and sensitivity
• Taqman probe technology

Specification

Extraction kit recommended for use:

Silica column extraction:

• • TopPURE® Fluid viral extraction kit
  • TopPURE® Genomic DNA extraction kit
  • TopPURE® Stool DNA Extraction Kit

Automatic magnetic beads extraction:

• TopPURE® Maga Genomic DNA/RNA Extraction Kit
• TopPURE® Maga Viral DNA/RNA Extraction Kit
• TopPURE® Manual Maga Viral DNA/RNA Kit

Read more: Instructional Video for Qualitative Real-time PCR Kit