Introducing TopSPEC® EHP qPCR Kit

Microsporidiosis causes slow growth in shrimp, leading to considerable economic losses. The disease is caused by a parasite. Enterocytozoon Hepatopenaei (EHP) is a parasite that infects the shrimp’s hepatopancreas, consuming nutrients from these organs and thereby slowing shrimp growth. In addition, it weakens the immune system and reduces disease resistance, making shrimp more susceptible to other serious infections such as white spot disease and acute hepatopancreatic necrosis disease (AHPND). EHP can persist in shrimp eggs, allowing vertical transmission from broodstock to offspring; therefore, early screening and detection are crucial.

TopSPEC® EHP qPCR Kit is a testing kit designed to detect the presence of Enterocytozoon Hepatopenaei (EHP) in aquaculture environments. Early detection of the pathogen helps minimize economic losses caused by EHP.

TopSPEC® EHP qPCR Kit

Highlights

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	Enterocytozoon Hepatopenaei (EHP) – SWP1 gene
Sample types	DNA after extraction
Input sample volume	200 µL
DNA volume	5 µL
Color channel detection	FAM: detect EHP HEX: internal control
Technology	TaqMan probe
PCR time	1.5h
Specificity	Only EHP is detected
Component	EHP qPCR mix, negative control, positive control, internal control(IC), Tube PCR.
Storage	12 months at -20^oC