INTRODUCTION
‘’Acute Hepatopancreatic Necrosis Disease’’ AHPND was initially referred to as "EMS’’ (Early Mortality Syndrome) caused by the bacterium Vibrio parahaemolyticus. Once inside the shrimp, this bacterium produces potent toxins that destroy tissue and disrupt hepatopancreatic function. This is also the main cause of early mortality in shrimp within 30–35 days after stocking, with a mortality rate of up to 70%.
TopSPEC® AHPND/EMS qPCR KIT is a diagnostic kit designed to detect the bacteria responsible for Acute Hepatopancreatic Necrosis Disease (AHPND) and Early Mortality Syndrome (EMS) in shrimp samples using Real-time PCR technology in aquaculture environments. The kit offers several outstanding advantages, including a simple and rapid workflow, high sensitivity, and excellent specificity.
TopSPEC® AHPND/EMS qPCR KIT
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Acute Hepatopancreatic Necrosis Disease/ Early Mortality Syndrome – PirA/B gene |
| Input sample | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Quantitative detection | FAM: detection of AHPND/EMS HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects AHPND/EMS |
| Storage | 12 months at -20oC |
| Kit components | EMS qPCR mix, negative control, positive control, internal control, PCR tube |
Recommended extraction kit
TopPURE® Highpure viral extraction kit (EXS-021)
TopPURE® Fluid Viral Extraction Kit (HI-712)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional Video for Qualitative Real-time PCR Kit
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