INTRODUCTION
Monkeypox disease is an infectious illness caused by the Monkeypox virus (MPXV), a member of the Orthopoxvirus genus. Common symptoms include skin rashes or mucosal lesions lasting 2–4 weeks, accompanied by fever, headache, muscle pain, back pain, fatigue, and lymphadenopathy. The disease is typically transmitted through contact with infected individuals or animals and can also be passed from mother to child. Therefore, early detection through diagnostic testing is crucial for effective treatment and prevention of transmission.
TopSENSI ® Monkeypox virus qPCR Kit enables qualitative detection of Monkeypox virus (MPXV) from swab samples collected from skin lesions or secretions using real-time PCR technology. The kit offers several key advantages, including a simple and rapid workflow, along with high sensitivity and specificity.
TopSENSI ® Monkeypox virus qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Monkeypox virus |
| Input sample | samples from secretions, surface swabs |
| Input sample volume | 200 µL |
| DNA volume | 10 µL |
| Quantitative detection | FAM: detect MPXV HEX: internal control Rnase P |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Sensitivity | 3.85 copies/reaction |
| Specificity | Only detect MPXV |
| Storage | 12 months at -20oC |
| Kit components | MPXV qPCR mix, negative control, positive control, internal control, tube PCR |
| Extraction kit is recommended | ||
| Silica column extraction | HI-112 | TopPURE® Genomic DNA Extraction Kit |
| HI-712 | TopPURE® Fluid Viral Extraction Kit | |
| Automatic magbead extraction | HI-612 | TopPURE® Maga Genomic DNA/RNA Extraction Kit |
| HI-007 | TopPURE® Maga Serum DNA/RNA Co-Extraction Kit | |
Read more: Instructional Video for Qualitative Real-time PCR Kit
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