Giới thiệu TopSPEC® WSSV – IHHNV qPCR KIT
White spot disease and subshell necrosis - hematopoietic organ are two common diseases that cause great damage in aquaculture, especially shrimp. White spot disease is caused bywhite spot syndrome virus (WSSV). The disease has a high rate of spread and mortality rate (80%). Meanwhile, subcapsular and hematopoietic necrosis is caused by the Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) virus. The disease causes shrimp death very quickly, 10 - 20 days after release. Therefore, early detection of pathogens present during the farming and care process is essential to limit damage caused by the disease.
TopSPEC® WSSV – IHHNV qPCR KIT là bộ kit multiplex real-time PCR phát hiện đồng thời bệnh đốm trắng và hoại tử cơ trên tôm. Bộ kit có nhiều ưu điểm nổi bật như quy trình đơn giản, nhanh chóng, độ nhạy và độ đặc hiệu cao.
TopSPEC® WSSV – IHHNV qPCR KIT
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | White spot syndrome virus (WSSV) và Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) – VP466 gene và NS2 gene |
| Input sample | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Quantitative detection | FAM: WSSV, TexasRed: IHHNV HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects WSSV and IHHNV |
| Storage | 12 months at -20oC |
| Kit components | WI qPCR mix, Chứng âm, Chứng dương WI, Chứng nội, tube PCR |
Recommended extraction kit
TopPURE® Highpure viral extraction kit (EXS-021)
Read more: Instructional Video for Qualitative Real-time PCR Kit
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