INTRODUCTION
White spot disease and subshell necrosis - hematopoietic organ are two common diseases that cause great damage in aquaculture, especially shrimp. White spot disease is caused bywhite spot syndrome virus (WSSV). The disease has a high rate of spread and mortality rate (80%). Meanwhile, subcapsular and hematopoietic necrosis is caused by the Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) virus. The disease causes shrimp death very quickly, 10 - 20 days after release. Therefore, early detection of pathogens present during the farming and care process is essential to limit damage caused by the disease.
TopSPEC® WSSV/IHHNV qPCR KIT is a multiplex real-time PCR kit that simultaneously detects white spot disease and myonecrosis in shrimp. The kit has many outstanding advantages such as a simple, fast process, high sensitivity and specificity.
TopSPEC® WSSV/IHHNV qPCR KIT
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | White spot syndrome virus (WSSV) và Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) – VP466 gene và Capsid protein gene |
| Input sample | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Quantitative detection | FAM: WSSV, TexasRed: IHHNV HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects WSSV and IHHNV |
| Storage | 12 months at -20oC |
| Kit components | WSSV/IHHNV qPCR mix, negative control, positive control, internal control(IC), Tube PCR. |
Recommended extraction kit
TopPURE® Highpure viral extraction kit (EXS-021)
Read more: Instructional Video for Qualitative Real-time PCR Kit
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