INTRODUCTION
Translucent post-larva disease (TPD) is a new disease in whiteleg shrimp (Penaeus vannamei), caused by Vibrio parahaemolyticus (VpTPD) causes and was first detected in China in 2020. The disease has an infection rate of about 60% and a high mortality rate in shrimp, especially PL4 - PL7 stage shrimp (90 – 100%). Typical disease symptoms in shrimp are usually pale or colorless hepatopancreas, shrunken and transparent body, and rapid death of shrimp. Therefore, early detection of pathogens in the farming environment will help significantly reduce damage caused by diseases.
TopSPEC® TPD qPCR Kit is a test kit designed to detect the presence of the vhvp-3 toxin gene in Vibrio strains causing TPD in shrimp using Real-time PCR technology.
TopSPEC® TPD qPCR Kit (SQS-107)
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Translucent post-larva disease (TPD) – vhvp-3 gene |
| Sample types | DNA after extraction |
| DNA volume | 5 µL |
| Color channel detection | HEX: vhvp-3 gene Cy5: internal control (IC) |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects vhvp-3 gene (causing TPD) |
| Component | TPD3 qPCR mix, negative control, positive control TPD3 , internal control, PCR tube |
| Storage | 12 months at -20oC |
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Read more: Instructional Video for Qualitative Real-time PCR Kit
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