Giới thiệu TopSPEC® TGEV RT-qPCR Kit
Transmissible Gastroenteritis of Swine is an infectious disease caused by the transmissible gastroenteritis virus (TGEV), transmitted through contaminated feed and farm environments. While the disease is typically mild in adult pigs, it poses a serious threat to piglets aged 1–10 days. Infected pigs may exhibit symptoms such as vomiting, anorexia, and diarrhea. Adult pigs tend to recover quickly, but piglets can experience mortality rates of up to 100% if not treated promptly. Notably, TGEV can persist in the bodies of sows for 2–3 months, making early detection critical for implementing timely and effective prevention and control strategies.
TopSPEC® TGEV RT-qPCR Kit is a diagnostic assay designed to detect the presence of TGEV in pigs using One-step RT-qPCR technology targeting the N gene of the virus. Early identification of the pathogen helps reduce the economic losses associated with TGEV infection.
TopSPEC® TGEV RT-qPCR Kit
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Transmissible Gastroenteritis of Swine (TGEV) – N gene |
| Sample types | Rectal swab sample, Intestinal (small intestine) tissue sample, stool sample, and environmental sample |
| Input sample volume | 200 µL |
| RNA volume | 5 µL |
| Color channel detection | FAM: detect N gene of TGEV HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects TGEV |
| Component | TGEV RT-qPCR mix, negative control, positive control, internal control (IC), PCR tube |
| Storage | 12 months at -20oC |
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Read more: Instructional Video for Qualitative Real-time PCR Kit
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