Introduction to the TopSPEC® Sesame Detection Kit
Food allergies are among the most pressing health concerns worldwide. Symptoms after consuming allergens include digestive disorders (diarrhea, vomiting), respiratory issues (rhinitis and asthma), skin reactions (urticaria, atopic dermatitis), and even anaphylactic shock, posing significant threats to both children's and adults' health and lives. Therefore, developing analytical methods for detecting allergens is crucial.
Sesame (Sesamum indicum) can be found in various food products such as bakery items (snacks, bread, biscuits), processed meats, fast foods, vegetarian foods, and traditional dishes. Sesame can also be consumed as cooking oil, with traces of sesame present in nearly all dishes from Eastern to Western cuisine. Sesame is considered one of the major causes of food allergies, and its incidence is increasing in many countries.
TopSPEC® Sesame Detection Kit is a diagnostic tool for detecting the presence of sesame allergens in food using Real-time PCR technology through the identification of 2S Albumin in sesame.
TopSPEC® Sesame Detection Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | 2S Albumin in sesame |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | FAM: Sesame HEX: internal control (IC) |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects 2S Albumin in sesame |
| Storage | 12 months at -20oC |
| Kit components | Sesame qPCR mix, negative control, positive control, internal control (IC), PCR tube |
| Recommended for extraction kit | ||
| Silica column extraction | TopPURE ® Plant DNA Extraction Kit | |
| TopPURE® Food DNA Extraction Kit | ||
Read more: Instructional Video for Qualitative Real-time PCR Kit
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