Giới thiệu TopSPEC® Rotavirus A/B/C RT-qPCR KIT
Rotavirus groups A, B and C are associated with enteritis, leading to diarrhea in piglets and weaned pigs, with group A being the most prevalent. These viruses are easily transmitted through direct contact. Infected pigs typically exhibit symptoms such as watery diarrhea, anorexia, emaciation, and lethargy. Co-infection with E. coli can exacerbate the condition, resulting in severe illness and potentially death. Rotaviruses are ubiquitous in swine farming environments, and without effective control measures, they can spread rapidly and cause outbreaks.
TopSPEC® Rotavirus A/B/C RT-qPCR KIT is a diagnostic assay designed for the detection of Rotavirus groups A, B, and C (RTVA, RTVB, RTVC) – targeting the VP6 gene in pigs and cattle using One-step RT-qPCR. It enables rapid identification of viral agents through a streamlined protocol, offering high sensitivity and specificity.
TopSPEC® Rotavirus A/B/C RT-qPCR KIT
Highlights
- Simple procedure
- PCR time is optimized for 2 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Rotavirus group A, B and C (RTVA, RTVB, RTVC) – VP 6 gene |
| Input sample | RNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | FAM: RTVA, Texas Red: RTVB, Cy5: RTVC targeting the VP6 gene HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects Rotavirus group A, B and C (RTVA, RTVB, RTVC) |
| Storage | 12 months at -20oC |
| Kit components | RTABC One-step qPCR mix, negative control, positive control, internal control (IC), tube PCR |
Extraction kit recommended for use:
Read more: Instructional Video for Qualitative Real-time PCR Kit
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