INTRODUCTION
Porcine circovirus 3 (PCV3) is a non-enveloped DNA virus belonging to the Circoviridae family. It has been detected in sows exhibiting dermatitis and nephropathy syndrome, as well as reproductive disorders such as fetal death. The virus increases mortality rates and reduces the reproductive success of pigs. Therefore, if not promptly and effectively detected, PCV3 can lead to significant economic losses in the swine farming industry. Therefore, PCV3 can cause significant economic losses in the swine industry if not detected promptly and accurately.
TopSPEC® PCV3 qPCR Kit is a molecular diagnostic assay designed to detect the presence of PCV3 – specifically targeting the ORF1 gene associated with disease in pigs – using Real-time PCR technology. The kit offers high sensitivity and specificity, making it suitable for both diagnostic and screening applications.
TopSPEC® PCV3 qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Porcine circovirus 3 (PCV3) – ORF1 gene |
| Input sample | Tissue samples (lymph nodes, liver, lungs); serum samples; environmental samples |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Quantitative detection | FAM: ORF1 gene of PCV3 HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects PCV3 |
| Storage | 12 months at -20oC |
| Kit components | PCV3 qPCR mix, negative control, positive control, internal control (IC), PCR tube |
Extraction kit recommended for use:
- TopPURE ® Genomic DNA extraction kit/ TopPURE® Fluid viral extraction kit recommended for extracting viral DNA from organ tissue-covered samples, environmental swab samples.
- TopPURE® Serum Viral DNA/RNA Co-Extraction Kit is recommended for viral DNA extraction from serum samples.
Read more: Instructional Video for Qualitative Real-time PCR Kit
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