About TopSPEC® Pasteurella multocida qPCR KIT
Pasteurella multocida is a Gram-negative bacterium belonging to the family Pasteurellaceae. It is the causative agent of hemorrhagic septicemia (HS), a highly fatal disease in pigs. Hemorrhagic septicemia can affect pigs of all ages but is most commonly observed in those between 3 and 6 months old. The pathogen primarily resides in the respiratory mucosa, and under adverse conditions—such as environmental changes or herd relocation—pigs may experience weakened immunity, allowing the bacteria to proliferate and cause disease.
TopSPEC® Pasteurella multocida qPCR Kit is a testing kit designed to detect the presence of Pasteurella multocida - KMT1 gene – associated with hemorrhagic septicemia in pigs using the qPCR technique. The kit utilizes a master mix containing UNG (Uracil-N-Glycosylase), which helps prevent cross-contamination from previous PCR products.
Illustration of Pasteurella multocida bacteria causing disease in pigs.
Highlights
- Simple procedure
- The optimized PCR duration is approximately 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Pasteurella multocida – KMT1 gene |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Color channel detection | FAM: KMT1 gene of Pasteurella multocida HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects Pasteurella multocida |
| Storage | 12 months at -20oC |
| Kit components | PM qPCR mix, negative control, PM positive control, internal control (IC), PCR tube |
Extraction kit recommended for use:
Silica column extraction:
Automatic magnetic beads extraction:
- TopPURE® Maga Genomic DNA/RNA Extraction Kit
- TopPURE® Maga Viral DNA/RNA Extraction Kit
- TopPURE® Manual Maga Viral DNA/RNA Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit
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