INTRODUCTION
Infectious Hypodermal and Hematopoietic Necrosis (IHHN) in shrimp is caused by the Infectious Hypodermal & Hematopoietic Necrosis Virus. Once infected, the virus destroys gland cells, lymphoid cells, and nerve cells, and it can spread both vertically and horizontally. Shrimp often die in large numbers 10-20 days after stocking. Adverse environmental factors combined with the presence of the virus exacerbate the damage significantly.
TopSPEC® IHHNV qPCR Kit is a testing kit designed to detect the presence of Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) in aquaculture environments. The kit features optimized primers and probes designed specifically for accurate detection of the virus. Early detection of the pathogen helps minimize economic losses caused by IHHNV.
TopSPEC® IHHNV qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Infectious Hypodermal & Hematopoietic Necrosi Virus (IHHNV) – Capsid protein gene |
| Input sample | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Quantitative detection | FAM: detection of IHHNV HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Only IHHNV is detected |
| Storage | 12 months at -20oC |
| Kit components | IHHNV qPCR mix, negative control, positive control, internal control(IC), Tube PCR. |
Recommended extraction kit
TopPURE® Highpure Viral Extraction Kit (EXS-021)
TopPURE® Fluid Viral Extraction Kit (HI-712)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional Video for Qualitative Real-time PCR Kit
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