INTRODUCTION
Hepatitis A virus (HAV) and norovirus (NoV) are the main agents that cause food poisoning in humans. HAV is a virus of the Picornaviridae family that causes hepatitis and Norovirus (norovirus) is a virus of the Caliciviridae family that causes occasional acute gastroenteritis. Currently, there is no conventional method to culture these viruses from food samples, so the currently recommended method is real-time PCR.
TopSPEC® HAV/NoV One-step RT-qPCR Kit is a test kit to detect hepatitis A virus (HAV) and Norovirus I and II (NoV) from food and animal surface samples. bivalve molluscs (BMS) using Real-time PCR technique. The kit has many outstanding advantages such as a simple, fast process, high sensitivity and specificity.
TopSPEC® HAV/NoV One-step RT-qPCR Kit
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Hepatitis A virus (HAV) – polyprotein gene, Norovirus I – ORF1 and VP1/ORF2 genes, and Norovirus II – RdRp gene |
| Input sample | RNA after extraction |
| Input sample volume | 200 µL |
| DNA/RNA volume | 5 µL |
| Quantitative detection | FAM: Norovirus I, II; Cy5: HAV HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects HAV and NoV |
| Storage | 12 months at -20oC |
| Kit components | HNoV One-step RT- qPCR mix, negative control, positive control (HAV, NoV), internal control, PCR tube |
Recommended extraction kit
Column extraction TopPURE® Fluid Viral Extraction Kit
Tách từ: TopPURE® Maga Genomic DNA/RNA Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit
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