About TopSPEC® CAdV qPCR Kit
Canine Adenovirus (CAdV), a double-stranded DNA virus from the Adenoviridae family, includes two major types: CAdV-1, which causes infectious canine hepatitis, and CAdV-2, associated with respiratory disease. Transmission occurs through direct contact with secretions such as urine, feces, and saliva, or indirectly via contaminated surfaces and objects. Puppies under one year of age and unvaccinated adult dogs are at highest risk, especially in cool, humid environments where the virus can persist despite common disinfectants. Early detection is essential for effective disease control and prevention.
TopSPEC ® CAdV qPCR Kit is a specialized diagnostic tool designed to detect Canine Adenovirus (CAdV) in dogs by targeting the hexon gene using Real-time PCR technology. Early identification of the pathogen facilitates timely intervention, thereby reducing the risk of severe clinical outcomes associated with CAdV infection.

TopSPEC ® CAdV qPCR Kit
Highlights
- Simple procedure
- The optimized PCR duration is approximately 1.5 hours
- High specificity and sensitivity
Specification
| Targets | Canine Adenovirus (CAdV) – hexon gene |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Color channel detection | FAM: CAdV HEX: CAdV Internal control |
| PCR time | 1.5h |
| Sensitivity | 4 copies/µL (20 copies/reaction) |
| Specificity | Kit only detects Canine Adenovirus (CAdV) |
| Storage | 12 months at -20oC |
Extraction kit recommended for use:
Silica column extraction:
- TopPURE® Viral DNA/RNA Extraction Kit
- TopPURE ® Stool DNA Extraction Kit
- TopPURE ® Fluid Viral Extraction Kit
Automatic magnetic beads extraction:
- TopPURE® Manual Maga Viral DNA/RNA Kit
- TopPURE® Maga Serum DNA/RNA Co-Extraction Kit
- TopPURE® Maga Viral DNA/RNA Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit




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