INTRODUCTION
Treponema pallidum (TP) is a Gram-negative bacterium belonging to the family Treponemataceae, and is the primary causative agent of syphilis and yaws in humans. Syphilis infections are frequently asymptomatic, which complicates accurate diagnosis and timely treatment. If left untreated, chronic infection can result in severe complications, particularly affecting the neurological and cardiovascular systems. In pregnant women, TP infection poses a significant risk of vertical transmission, potentially leading to neonatal conditions such as arthritis, blindness, deafness, and meningitis. Therefore, early screening and detection of syphilis are critically important.
TopSENSI® Treponema pallidum qPCR kit is used to detect the presence of Treponema pallidum The test is performed using Real-time PCR technology on DNA extracted from various human clinical specimens. The results obtained from this diagnostic kit are intended for clinical reference only and should not be used as the sole basis for diagnosis or exclusion of disease.
TopSENSI® Treponema pallidum qPCR kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Treponema pallidum (TP) |
| Sample types | Sample for vaginal or rectal swab |
| Input sample volume | 200µL |
| DNA volume | 10µL |
| Color channel detection | FAM: detect TP agent HEX: internal control |
| Sensitivity | 20,00 copies/reaction |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Only detect TP agents |
| Component | HPV qPCR mix, Negative control, Positive control, Internal control (IC), Tube PCR. |
| Storage | 12 months at -20oC |
Recommended extraction kit
- TopPURE® Genomic DNA Extraction Kit (HI-112)
- TopPURE® Fluid Viral Extraction Kit (HI-712)
- TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional Video for Qualitative Real-time PCR Kit
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