About TopSENSI ® STD-12 qPCR Kit
Sexually transmitted diseases (STDs) are among the most common causes of illness worldwide (WHO) and are ranked among the top ten diseases for which adults in developing countries seek health care services.
STDs are a major public health issue, not only because of their severe complications and long-term sequelae on health, but also because they significantly increase the risk of human immunodeficiency virus (HIV) transmission by 5-10 times compared to uninfected individuals. Additionally, STDs impose significant economic burdens on patients.
STDs can be acquired due to a lack of information about STD transmission, improper personal hygiene leading to infections, or neglecting necessary preventive measures during sexual intercourse.
70 to 80% of STDs share similar symptoms despite originating from different pathogens; in fact, many STDs are asymptomatic and difficult to detect. Therefore, screening is crucial for early detection and appropriate treatment to control the spread of these diseases.
Single PCR tests for STDs typically have high specificity for each pathogen, but the limitation of this method is the time-consuming process of sequentially testing each pathogen with different kits to obtain results. This study aims to develop a kit that simultaneously detects 12 pathogens, including Mycoplasma hominis, Mycoplasma genitalium, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum, Gardnerella vaginalis, Human herpesvirus 1, Human herpesvirus 2, Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, and Candida albicans, in samples using multiplex real-time PCR.
TopSENSI ® STD-12 qPCR Kit is used to detect the presence of DNA from 12 common sexually transmitted disease (STD) agents in various human samples using real-time PCR technology. The test results of this kit are intended for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.
TopSENSI ® STD-12 qPCR Kit
HIGHLIGHTS
- Simple process easy manipulation
- The PCR process is completed rapidly in approximately 50 minutes.
- High specificity and sensitivity
- Taqman probe technology
SPECIFICATIONS
| Targets | Mycoplasma hominis, Mycoplasma genitalium, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum, Gardnerella vaginalis, Human herpesvirus 1, Human herpesvirus 2, Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, and Candida albicans, |
| Input sample | Vaginal/urethral swab/cervical biopsy |
| Extracted sample volume | 200µL |
| PCR sample volume | 10µL |
| Color channel detection | FAM, HEX, TexasRed and Cy5 |
| Sensitivity | 10,00 – 40,00 copies/reaction |
| Technology | TaqMan probe |
| PCR time | 50 minutes |
| PCR temperature cycling profile | 1 cycle: 95°C – 60s 45 cycles: 95°C – 10s 60°C - 30s (signal reading) |
| Component | STD-12 qPCR Mix (A), STD-12 qPCR Mix (B), STD-12 qPCR Mix (C), STD-12 qPCR Mix (D), Negative control, STD-12 Positive control, Internal control (IC) |
| Storage | -20°C, 6 months from the date of manufacture |
RECOMMENDED FOR THE EXTRACTION KITS:
Read more: EZ01 intructions for use
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