INTRODUCTION
Human papillomavirus (HPV) is the main cause of cervical cancer, affecting nearly 500,000 women around the world every year and killing more than 270,000. The disease usually progresses very slowly and until there are signs that often cause serious consequences for the health of women, so early detection and screening of this virus is extremely urgent.
TopSENSI ® HPV PCR-RDB Genotype Kit is a testing kit designed to determine HPV genotypes using a combination of Polymerase Chain Reaction (PCR) and Reverse Dot Blot (RDB) methods. The kit enables accurate detection of the presence of 35 HPV (Human papillomavirus) genotypes in humans, including 14 high-risk strains, 8 probable high-risk strains, and 13 low-risk strains.
HIGHLIGHTS
- Simple process easy manipulation
- simultaneously detects 35 types of variants including high-risk, low-risk and high-risk
- High specificity
- Convenience: providing both PCR and RDB kits
COMPONENTS
The kit includes 1 box of HPV PCR Kit and 1 box of HPV RDB Kit
SPECIFICATIONS
- HPV qualitative
| Kit | HPV PCR Kit |
| Targets | Qualitative of Human Papillomavirus (HPV) |
| Input sample | Vaginal/urethral swab samples or cervical biopsy specimens |
| Extracted sample volume | 200µL |
| DNA volume | 10µL |
| PCR time | 1 hour 45 minutes |
| Specificity | Only HPV is detected |
| Component | HPV PCR mix, Negative control, Positive control, PCR tube. |
| Storage | 6 months at 20oC |
- HPV genotype detection
| Targets | Genotyping of Human Papillomavirus (HPV) |
| Input sample | PCR products |
| Input sample volume | 25µL |
| Type | 35 types of HPV |
| Technology | Reverse Dot Blot (RDB) |
| Time | 3-4 hours |
| Component | RDB buffer (1, 2, 3, 4, 5), TMB buffer, membrane, plastic box |
| Storage | 6 months at a storage temperature of 2–8 °C (with plastic containers specifically stored at 15–25 °C) |
Recommended for extraction kit
| Silica column extraction | TopPURE® Genomic DNA Extraction Kit |
| TopPURE® Fluid Viral Extraction Kit | |
| Automatic magbead extraction | TopPURE® Maga Genomic DNA/RNA Extraction Kit |
Illustrative RDB results
Positions of HPV genotypes on the hybridization membrane (A) and genotyping results from clinical samples (B)
- 14 High-Risk HPV Genotypes (Red): 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68.
- 8 Possibly High-Risk HPV Genotypes (Yellow): 26, 34, 53, 67, 69, 70, 73, 82.
- 13 low-risk HPV genotypes (Green): 6, 11, 40, 42, 43, 44, 54, 61, 62, 71, 72, 8
- Positive control: RP gene
Read more: Instructional Video for Qualitative Real-time PCR Kit
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