HPV introduction
Human Papillomavirus (HPV) is a common sexually transmitted DNA virus. It is recognized as the etiological agent of a spectrum of diseases in both males and females, including precancerous lesions that may progress to malignancy and the formation of genital warts.
Although the majority of HPV infections are asymptomatic and resolve spontaneously, persistent infection can lead to pathology. In women, chronic infection with specific high-risk genotypes—most notably types 16 and 18—can induce precancerous cervical lesions that, if untreated, may advance to invasive cervical carcinoma. HPV is also implicated in oropharyngeal and anogenital cancers as well as other disease entities in both sexes.
More than 100 HPV genotypes have been identified, each designated by a numerical identifier. Certain low-risk genotypes cause anogenital warts but lack oncogenic potential. However, nearly all cases of cervical cancer (approximately 99 percent) are attributable to genital HPV infection. Fourteen high-risk genotypes are classified as carcinogenic—types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68—with types 16 and 18 together accounting for roughly 70 percent of global cervical cancer cases.
TopSENSI ® HPV High Risk & 6,11 qPCR Kit is designed for the simultaneous genotyping of fourteen high-risk human papillomavirus (HPV) types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and the qualitative detection of two common low-risk types (HPV 6 and 11) in vaginal or urethral swab specimens and cervical biopsy samples. The assay employs real-time polymerase chain reaction technology, utilizing genotype-specific target gene sequences to ensure precise identification.
The kit offers several advantages: high specificity, high sensitivity, and an internal control system that helps monitor sample collection, extraction, and amplification. The test results from this kit are intended for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.
HIGHLIGHTS
- Simple process easy manipulation
- The time to perform PCR is fast, only about 1 hour and 30 minutes
- High specificity and sensitivity
- Taqman probe technology
- Many HPV genotypes were detected.
TopSENSI ® HPV High Risk & 6,11 qPCR Kit (SQH-017)
SPECIFICATIONS
| Targets | Genotyping of 14 high-risk HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and qualitative detection of 2 common low-risk types (HPV 6 and 11). |
| Input sample | Vaginal/urethral swab samples or cervical biopsy specimens |
| Extraction sample volume | 200 µL |
| PCR sample volume | 10 µL |
| Fluorescent channels | FAM, HEX, TexasRed, and Cy5 used for corresponding targets |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Analytical Specificity | The kit detects only the specific target HPV types with high specificity |
| Storage | 12 months at -20oC |
| Kit components | qPCR Mix, HPV Genotype A, HPV Genotype B, HPV Genotype C, HPV Genotype D, Chứng dương HR & 6, 11, Chứng âm, PCR tube |
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Read more: Instructional Video for Qualitative Real-time PCR Kit
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