About TopSENSI ® STD-14 qPCR Kit
Sexually Transmitted Diseases (STDs) refer to a group of infections caused by bacteria, viruses, parasites, or fungi. These infections are primarily transmitted through unprotected sexual contact (vaginal, anal, or oral). Additionally, certain STDs can be transmitted via blood-to-blood contact or vertically from mother to child during pregnancy and childbirth. Many STD infections remain asymptomatic, which escalates the risk of transmission and complicates early detection. If left undiagnosed or untreated, STDs can lead to severe clinical complications, including infertility, pelvic inflammatory disease, increased susceptibility to HIV, and anogenital cancers.
TopSENSI ® STD-14 qPCR Kit is developed for the simultaneous detection of 14 high-risk genital pathogens, including: Mycoplasma hominis; Mycoplasma genitalium; Trichomonas vaginalis; Streptococcus agalactiae; Ureaplasma parvum; Ureaplasma urealyticum; Gardnerella vaginalis; Human herpesvirus 1; Human herpesvirus 2; Treponema pallidum; Haemophilus ducreyi; Chlamydia trachomatis; Neisseria gonorrhoeae and Candida albicans The test is performed using Real-time PCR technology on DNA extracted from various human clinical specimens. The results obtained from this diagnostic kit are intended for clinical reference only and should not be used as the sole basis for diagnosis or exclusion of disease.
TopSENSI ® STD-14 qPCR Kit
HIGHLIGHTS
- Rapid PCR execution (< 2 hours)
- High specificity and sensitivity
- Taqman probe technology
- Detection of multiple targets in a single qPCR reaction.
SPECIFICATIONS
| Targets | The kit is designed for the detection of 14 high-risk genital pathogens, including: Mycoplasma hominis; Mycoplasma genitalium; Trichomonas vaginalis; Streptococcus agalactiae; Ureaplasma parvum; Ureaplasma urealyticum; Gardnerella vaginalis; Human herpesvirus I; Human herpesvirus 2; Treponema pallidum; Haemophilus ducreyi; Chlamydia trachomatis; Neisseria gonorrhoeae and Candida albicans |
| Input sample | Vaginal/urethral swab or cervical biopsy specimens. |
| PCR sample volume | 10 µL |
| Fluorescent channels | FAM, HEX, TexasRed và Cy5: for targets |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Analytical Specificity | Kit provides exclusive specificity for 14 target urogenital pathogens, with zero cross-reactivity against human genomic DNA or common microorganisms. |
| Storage | 12 months at -20oC |
| Kit components | STD-14 qPCR Mix, Primer/probe A Mixture, Primer/probe B Mixture, Primer/probe C Mixture, Primer/probe D Mixture, Positive control, Negative control, Internal control (IC), PCR tube |
Analytical sensitivity
| Group | Targets | Sensitivity (copies/µL) |
| A | Mycoplasma hominis | 1,50 |
| Mycoplasma genitalium | 1,00 | |
| Trichomonas vaginalis | 1,00 | |
| Streptococcus agalactiae | 1,00 | |
| B | Ureaplasma parvum | 1,50 |
| Ureaplasma urealyticum | 1,50 | |
| Gardnerella vaginalis | 1,00 | |
| C | Chlamydia trachomatis | 4,00 |
| Neisseria gonorrhoeae | 4,00 | |
| Candida albicans | 2,50 | |
| Haemophilus ducreyi | 1,50 | |
| D | Human herpesvirus I | 1,00 |
| Human herpesvirus II | 3,50 | |
| Treponema pallidum | 2,00 |
Recommended extraction kit
| Silica column extraction | TopPURE® Genomic DNA Extraction Kit |
| TopPURE ® Fluid Viral Extraction Kit | |
| TopPURE® Serum Viral DNA/RNA Co-Extraction Kit | |
| Automatic magbead extraction | TopPURE® Maga Genomic DNA/RNA Extraction Kit |
| TopPURE® Maga Serum DNA/RNA Co-Extraction Kit |
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