CTAB SOLUTION

Specifications

Cetyltrimethylammonium bromide (CTAB) is a non-ionic detergent capable of precipitating nucleic acids and polysaccharides. CTAB exhibits reversible binding affinity to DNA and polysaccharides. Specifically, under high salt concentrations (e.g., 1.4 M NaCl present in the extraction buffer), CTAB interacts with both DNA and polysaccharides. However, the CTAB–DNA complex remains soluble, whereas the CTAB–polysaccharide complex precipitates. Following cell lysis using CTAB, DNA is isolated and purified with the aid of chloroform, isopropanol, and 70% ethanol solutions. isopropanol, ethanol 70%.

Cetyltrimethylammonium bromide (CTAB Solution)

Manual instruction

Reference Protocol for Plant DNA Extraction Using CTAB Solution:

• Grind 5 g of plant tissue using a mortar and pestle in liquid nitrogen.
• Add 500 μL of CTABsolution and mix thoroughly.
• Incubate the sample at 65°C for 30 minutes.
• Add 200 μL of Chloroform: Isoamylalcohol 24:1 and mix vigorously.
• Centrifuge at 13,000 rpm for 5 minutes. Carefully transfer 500 μL of the upper aqueous phase to a new tube.
• Add an equal volume of ice-cold absolute isopropanol (2–8°C) and incubate at –20°C for 10 minutes.
• Centrifuge at 13,000 rpm for 10 minutes.
• Discard the supernatant, wash the pellet with 500 μL of 70% ethanol, and allow the DNA to air dry completely.
• Resuspend the DNA in 50 μL of TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0)

Precautions / Warnings

• Personal protective equipment (PPE) such as gloves, masks, and respirators should be worn when handling chemicals.
• Exercise caution when using liquid nitrogen and chloroform:isoamyl alcohol (24:1).
• Liquid nitrogen is flammable and may cause cold burns.

Read more:

• Other Bio-reagents
• Manual instruction