HPV introduction
Human Papillomavirus (HPV) is a common sexually transmitted DNA virus. It is recognized as the etiological agent of a spectrum of diseases in both males and females, including precancerous lesions that may progress to malignancy and the formation of genital warts.
Although the majority of HPV infections are asymptomatic and resolve spontaneously, persistent infection can lead to pathology. In women, chronic infection with specific high-risk genotypes—most notably types 16 and 18—can induce precancerous cervical lesions that, if untreated, may advance to invasive cervical carcinoma. HPV is also implicated in oropharyngeal and anogenital cancers as well as other disease entities in both sexes.
More than 100 HPV genotypes have been identified, each designated by a numerical identifier. Certain low-risk genotypes cause anogenital warts but lack oncogenic potential. However, nearly all cases of cervical cancer (approximately 99 percent) are attributable to genital HPV infection. Fourteen high-risk genotypes are classified as carcinogenic—types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68—with types 16 and 18 together accounting for roughly 70 percent of global cervical cancer cases.
ABT® DNA Genotype qPCR Kit is a diagnostic assay designed for qualitative detection of HPV DNA from 14 high-risk genotypes, with specific identification of genotypes 16 and 18. Samples are extracted from cervical swab or biopsy specimens and analyzed using Real-time PCR technology. Notably, the kit incorporates an endogenous RP internal control to effectively monitor sample collection, nucleic acid extraction, and PCR amplification processes. The results obtained from this diagnostic reagent are intended for clinical reference only and must not be used as the sole basis for diagnosis or exclusion of disease.
Advantages
- Simple process easy manipulation
- The time to perform PCR is fast, only about 1 hour and 30 minutes
- High specificity and sensitivity
- Taqman probe technology
ABT® DNA Genotype qPCR Kit
SPECIFICATIONS
| Targets | HPV High-risk, 16 & 18 genotype |
| Extraction sample type | Cervical smear or biopsy |
| Extraction sample volume | 200 µL |
| PCR sample volume | 10 µL |
| Fluorescent channels | FAM: HPR-HR, HPV-18 Texasred: HPV-16 HEX: internal control (RNase P) |
| Technology | Taqman probe |
| Analytical sensitivity | High risk: 1.77 copies/µL (17.7 copies/reaction) Genotype 16: 1.03 copies/µL (10.3 copies/reaction) Genotype 18: 0.56 copies/µL (5.6 copies/reaction) |
| PCR time | 1.5h |
| Specificity | The kit only specifically detects 14 high-risk HPV types and specifically detects types 16 and 18. |
| Storage | 12 months at -20oC |
| Kit components | HPV HR qPCR mix, HPV-16,18 qPCR Mix, positive control, negative control, PCR Tube |
Recommended extraction kit
| Silica column extraction | TopPURE® HPV DNA Extraction Kit |
| TopPURE® Fluid Viral Extraction Kit | |
| TopPURE® Genomic DNA Extraction Kit | |
| Automatic magbead extraction | TopPURE® Maga Genomic DNA/RNA Extraction Kit |
Read more: Instructional Video for Qualitative Real-time PCR Kit
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