Giới thiệu TopSPEC® TGEV RT-qPCR Kit

Transmissible Gastroenteritis of Swine is an infectious disease caused by the transmissible gastroenteritis virus (TGEV), transmitted through contaminated feed and farm environments. While the disease is typically mild in adult pigs, it poses a serious threat to piglets aged 1–10 days. Infected pigs may exhibit symptoms such as vomiting, anorexia, and diarrhea. Adult pigs tend to recover quickly, but piglets can experience mortality rates of up to 100% if not treated promptly. Notably, TGEV can persist in the bodies of sows for 2–3 months, making early detection critical for implementing timely and effective prevention and control strategies.

TopSPEC® TGEV RT-qPCR Kit is a diagnostic assay designed to detect the presence of TGEV in pigs using One-step RT-qPCR technology targeting the N gene of the virus. Early identification of the pathogen helps reduce the economic losses associated with TGEV infection.

TopSPEC® TGEV RT-qPCR Kit

Highlights

Simple procedure
PCR time was optimized for 2 hours
Various input sample types
High specificity and sensitivity

Specification

Targets	Transmissible Gastroenteritis of Swine (TGEV) – N gene
Sample types	Rectal swab sample, Intestinal (small intestine) tissue sample, stool sample, and environmental sample
Input sample volume	200 µL
RNA volume	5 µL
Color channel detection	FAM: detect N gene of TGEV HEX: internal control
Technology	TaqMan probe
PCR time	1 hour 45 minutes
Specificity	Kit only detects TGEV
Component	TGEV RT-qPCR mix, negative control, positive control, internal control (IC), PCR tube
Storage	12 months at -20^oC