INTRODUCTION
Shrimp necrosis under the shell and hematopoietic organs caused by the Infectious Hypodermal & Hematopoietic Necrosis virus. This virus, after infection, will destroy glandular cells, lymphatic system, and nerve cells, and in particular, it can spread both vertically and horizontally. Shrimp often die 10-20 days after stocking. When adverse environmental factors combine with pathogens, they will cause much greater damage.
TopSPEC® IHHNV qPCR Kit is a testing kit designed to detect the presence of Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) in aquaculture environments. Early detection of the pathogen helps reduce economic losses caused by IHHNV.
TopSPEC® IHHNV qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Infectious Hypodermal & Hematopoietic Necrosis (IHHNV) – NS2 gene |
| Input sample | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Color channel detection | FAM: detect IHHNV HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Only IHHNV is detected |
| Component | IHHNV qPCR mix, negative control, positive control, internal control(IC), Tube PCR. |
| Storage | 12 months at -20oC |
Recommended extraction kit
TopPURE® Highpure viral extraction kit (EXS-021)
TopPURE® Fluid Viral Extraction Kit (HI-712)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional Video for Qualitative Real-time PCR Kit









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