About TopSPEC® TiLV RT-qPCR Kit

Tilapia Lake Virus (TiLV) is an infectious pathogen that causes disease across all life stages of both farmed and wild tilapia. Clinical signs are generally nonspecific and include lethargy, anorexia, ocular alterations (such as corneal opacity, exophthalmia, and cloudiness), dermatological changes (erosions, hemorrhages, and darkened pigmentation), abdominal distension/ascites, and scale loss. TiLV can be detected in multiple organs, including the gills, spleen, anterior kidney, liver, brain, and heart. Transmission occurs both horizontally (via contact, mucus, feces, and water) and vertically (from broodstock to eggs or milt).

TopSPEC® TiLV RT-qPCR Kit is designed to detect the presence of Tilapia Lake Virus by targeting the hypothetical protein gene, which causes disease in tilapia, using RT-qPCR technology.

TopSPEC® TiLV RT-qPCR Kit

Highlights

Simple procedure
Optimize the PCR time to 1.5 hours.
Various input sample types
High specificity and sensitivity

Specification

Targets	Tilapia Lake Virus – Hypothetical protein gene
Sample types	RNA after extraction
DNA volume	5 µL
Color channel detection	FAM: TiLV HEX: internal control (IC)
Technology	TaqMan probe
PCR time	1 hour 45 minutes
Specificity	Kit only detects TiLV
Component	TiLV RT-qPCR Mix, Negative control, Positive control, Internal control (IC), PCR tube
Storage	12 months at -20^oC