About TopSPEC® NOS Terminator qPCR Kit
Nopaline synthase terminator gene (NOS Terminator) is a transcription terminator widely used in gene synthesis. This sequence originates from the bacterium Agrobacterium tumefaciens in soil. This bacterium has the ability to insert DNA into plants. Based on this natural characteristic of A. tumefaciens, it has been widely used for artificial gene transfer in plants. After the gene transfer process, the NOS Terminator sequence remains and exists in the genome of the transgenic plant. Therefore, detecting this sequence demonstrates the presence of genetic modification in the tested plant.
TopSPEC® NOS Terminator qPCR Kit is a diagnostic kit designed to detect genetically modified material by identifying the presence of the NOS terminator (Nopaline synthase terminator gene) – a regulatory element widely used in genetically modified organisms – in plants and other food products.
TopSPEC® NOS Terminator qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Nopaline synthase terminator gene (NOS Terminator) |
| Sample types | DNA after extraction from plant and food samples |
| Sample volume | 5µL |
| Color channel detection | FAM: detects NOS Terminator HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects NOS Terminator |
| Component | TNOS qPCR mix, negative control, positive control, internal control (IC), PCR tube |
| Storage | 12 months at -20oC |
Recommended extraction kit
TopPURE® Plant DNA Extraction Kit
TopPURE® Food DNA Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit
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