INTRODUCTION
Influenza virus , commonly known as flu viruses, belong to the Orthomyxoviridae family and are classified into three types: A, B, and C. Among these, Influenza A and B viruses are the primary agents responsible for seasonal epidemics each year, characterized by rapid transmission. Influenza A virus can infect humans, poultry (such as birds and chickens), and livestock (including pigs and horses), whereas Influenza B virus is restricted to human hosts. Due to their high transmissibility and potential to cause pandemics, early diagnosis and preventive measures are essential for controlling influenza outbreaks.
TopSPEC® Influenza virus A/B RT-qPCR KIT is a molecular diagnostic assay designed to detect the presence of Influenza A and B viruses by targeting the M and HA genes using One-step Real-time RT-PCR. The kit enables rapid detection through a streamlined protocol, offering high sensitivity and specificity.
TopSPEC® Influenza virus A/B RT-qPCR KIT
Highlights
- Simple procedure
- PCR time is optimized for 2 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Influenza virus A and B – M gene, HA gene |
| Input sample | RNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | FAM: M gene of FluA TEXAS RED: HA gene of FluB HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects Influenza virus A/B |
| Storage | 12 months at -20oC |
| Kit components | FluA/B RT-qPCR mix, chứng âm, chứng dương, chứng nội, tube PCR |
Extraction kit recommended for use:
Read more: Instructional Video for Qualitative Real-time PCR Kit
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