Giới thiệu TopSPEC ® H5N1 RT-qPCR Kit
The avian influenza A/H5N1 is a prevalent disease in poultry, affecting birds, chickens,... The incubation period ranges from several hours to 28 days, making it challenging to determine. Infected birds exhibit symptoms such as high fever, lethargy with ruffled feathers, huddling together, swollen bloodshot eyes, darkened comb, and hemorrhaging in the legs. Avian influenza A/H5N1 is one of the most dangerous diseases in poultry, with a 100% mortality rate and rapid transmission. Immediate measures such as culling and quarantine are crucial upon disease detection.
TopSPEC ® H5N1 RT-qPCR Kit is a diagnostic assay designed to detect the presence of Influenza A/H5N1 virus – specifically targeting the HA and NA genes – in poultry using One-step RT-qPCR. Early detection of the pathogen is essential for preventing the severe consequences associated with H5N1 infection.

TopSPEC ® H5N1 RT-qPCR Kit
Highlights
- Simple procedure
- PCR time is optimized for 2 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Virus cúm A/H5N1 – HA gene và NA gene |
| Input sample | RNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | TexasRed: detect HA gene of H5;
Cy5: detect NA gene of N1 HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects H5N1 |
| Storage | 12 months at -20oC |
| Kit components | H5N1 RT-qPCR mix, chứng âm, chứng dương (H5, N1), chứng nội, tube PCR |
Extraction kit recommended for use:
Read more: Instructional Video for Qualitative Real-time PCR Kit








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