TopSPEC® Flavobacterium columnare qPCR KIT (SQF-101)

About TopSPEC® Flavobacterium columnare qPCR KIT

Pangasius (Pangasianodon hypophthalmus) is one of the main freshwater aquaculture species, serving as an important source of food and exportable seafood in Vietnam and other tropical countries such as Thailand, Cambodia, Myanmar, Indonesia, Malaysia, Bangladesh, India, and the Philippines. However, the shift from extensive to intensive farming has reduced available culture areas and increased environmental pollution, creating favorable conditions for the emergence of infectious diseases in Pangasius.

White Tail Disease, tail rot, or columnaris disease is a common and highly damaging disease in Pangasius, particularly affecting small-sized fish. Mortality can be very high within just a few days of infection, especially after fish are transported and stocked. Clinical signs can be recognized through behavior and the appearance of white spots on the body.In the early stage, infected fish often swim slowly and aimlessly, secrete excessive mucus, and develop numerous white spots on the body. Feeding decreases gradually until the fish stop eating altogether. Fish become increasingly lethargic, with stiffening tails that progressively affect the body until they stop swimming, float horizontally on the water surface, and are often blown by the wind toward the pond edges in clusters.In the late stage, mucus is lost, the skin feels dry and rough to the touch, gill necrosis occurs, and white spots appear on the skin and dorsal fins, spreading from the dorsal fin toward the tail. The tail fin may show hemorrhagic spots, tears, and breakage, and in severe cases, ulcers develop, the tail may be eroded or partially lost, and lesions can extend into the muscle. White Tail and tail rot disease are particularly dangerous for fish aged 20–30 days, causing high losses. The disease progresses rapidly, with onset of symptoms and mortality occurring within 2–3 days. Mortality rates are extremely high, typically ranging from 60–90%.The causative agent of this disease has been identified as Flavobacterium columnare (FC) is the causative agent. This is a thin, rod-shaped, Gram-negative bacterium belonging to the family Flavobacteriaceae it was first isolated in 1922 by Davis and colleagues and its name was officially confirmed by Bernardet and colleagues in 1989. Currently, F. columnare has been reported worldwide. The bacterium typically proliferates and causes disease at temperatures above 20^oC, however, there have been reports of this bacterium causing disease in salmon at water temperatures as low as 12–15°C.^oC. The virulence mechanisms of F. columnare are not yet fully understood, and effective control and preventive measures are still lacking.

TopSPEC® Flavobacterium columnare qPCR Kit is a testing kit designed to detect the presence of Flavobacterium columnare causing White Tail Disease in Pangasius (tra fish), detected using Real-time PCR technology.

TopSPEC® Flavobacterium columnare qPCR Kit

Highlights

• Simple procedure
• Optimize the PCR time to 1.5 hours.
• Various input sample types
• High specificity and sensitivity

Specification

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Read more: Instructional Video for Qualitative Real-time PCR Kit