Introduction TopSPEC® ADV qPCR Kit
Pseudorabies (PR) , also known as Aujeszky’s Disease (AD), is a viral disease that affects pigs and other animals such as dogs, cats, and cattle. It is caused by the Pseudorabies virus (PRV), a member of the Herpesviridae family. ADV infection in pigs leads to clinical symptoms including fever, anorexia, respiratory distress, and neurological signs. Infected pigs may also exhibit behavioral changes such as aggression or depression. The virus can be transmitted among pigs through direct contact with infected animals, as well as indirectly via contaminated equipment, feed, and clothing. Although ADV is a zoonotic disease, transmission to humans is extremely rare.
TopSPEC® ADV qPCR Kit s a diagnostic tool designed for the detection of ADV (PRV) – specifically the Glycoprotein B gene – in pigs using Real-time PCR technology. Early detection of the pathogen is critical for preventing the severe consequences associated with ADV infection.
TopSPEC® ADV qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Targets | Aujeszky’s, Pseudorabies (ADV-PRV) – Glycoprotein B gene |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Quantitative detection | FAM: Glycoprotein B gene of ADV HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detect |
| Storage | 12 months at -20oC |
| Kit components | ADV qPCR mix, negative control, positive control, internal control (IC), PCR tube |
| Recommended for extraction kit | ||
| Silica column extraction | TopPURE® Genomic DNA Extraction Kit | |
| TopPURE® Blood DNA Extraction Kit | ||
| TopPURE® Serum Viral DNA/RNA Co-Extraction Kit | ||
Read more: Instructional Video for Qualitative Real-time PCR Kit
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