TopSENSI® Shigella qPCR Kit (RUO) (SQH-145)

Giới thiệu TopSENSI ® Shigella qPCR Kit

Shigella are Gram-negative, non-motile, facultatively anaerobic bacilli that do not form spores. They are the causative agents of bacillary dysentery (with 25% of cases showing clear dysentery symptoms, 25% being asymptomatic, and the majority presenting with mild diarrhea, with a few chronic cases). In Vietnam, the most common Shigella pathogens are S. flexneri (61.5%) and S. sonnei (38.5%). Shigella outbreaks typically occur in densely populated areas with poor sanitation. Bacillary dysentery is very common in children, though adults can also contract the disease, usually with less severity. Shigella invades the mucosa of the colon, causing mucus secretion, congestion, leukocyte infiltration, edema, and often superficial mucosal ulceration. Shigella dysenteriae type 1 produces Shiga toxin, which causes diarrhea and sometimes hemolytic - uremic syndrome.

Shigella

TopSENSI ® Shigella qPCR Kit is used to detect the presence of Shigella DNA in samples extracted from various human samples using Real-time PCR technology. The results of this test kit are for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.

Advantages of using the kit detects Shigella

• Simple procedure
• PCR time was optimized for 1 hour 15 minutes
• Various input sample types
• High specificity and sensitivity

Specification 

Recommended extraction kit 

TopPURE® Genomic DNA Extraction Kit (HI-112)

TopPURE® Stool DNA Extraction Kit (HI-192) 

Read more: Instructional Video for Qualitative Real-time PCR Kit Utilization