TopPURE® FFPE DNA EXTRACTION KIT CE (HI-182)

INTRODUCTION

FFPE is a cancer biopsy tissue sample, fixed with formalin and molded paraffin to help preserve tissue structure intact for a long time. This is also a great resource for retrospective research on cancer, histopathology, and molecular genetics. Currently, gene sequencing, Realtime PCR are often used to accurately detect gene mutations, quantify cancer gene expression, etc. Therefore, DNA extraction from FFPE tissue is an important first step.

TopPURE® FFPE DNA Extraction Kit được sản xuất dựa trên công nghệ cột silica theo tiêu chuẩn ISO 9001 và ISO 13485 cho phép thu nhận DNA chất lượng cao.

HIGHLIGHTS

• Safety - Solvents are not toxic
• The quality of the resulting DNA has a high integrity, helping to increase PCR amplification of large segments
• Follow to silica column principle: easy, convenient
• Efficiency: able to collect 12ug
• Completed solution: include Paraffin removal step and DNA extraction

SPECIFICATIONS

• Sample type: FFPE
• Input sample volume: 8 samples with 5-10µm thick
• Efficiency: up to 12µg
• Extraction time: 3 hour for 10 samples
• Purity:OD260/280 ~ 1.7-2.2
• Storage: Buffers can be stored dry at room temperature. Proteinase K and RNA Carrier are stored at 2-8^oC
• Expiration date: 12 months from the date of manufacture

COMPONENTS

DATA EXPERIMENT 

Figure 1 PCR results of extracted products for βactin gene with varying fragment sizes

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