INTRODUCTION
TopPURE® BLOOD DNA EXTRACTION KIT is manufactured based on silica column membrane technology according to ISO 9001 and ISO 13485 standards, allowing high-quality DNA collection from whole blood samples containing anticoagulants (EDTA, citrate). The kit is designed with a simple, fast, and safe process in mind. The results of obtaining purified DNA are applied directly in experiments: Real-time PCR, sequencing, southern blot, SNP,...
HIGHLIGHTS
- Process for rapid extraction of whole blood samples from humans or animals
- High quality purification
- No organic solvent or toxic reagent (Phenol, Chloroform)
- Convenient: Provide a complete all chemical without needing to add chemicals or materials outside
- The kit includes Ethanol for mixing Wash Buffer 1 and Wash Buffer 2
SPECIFICATIONS
- Input sample types: Whole blood
- Input sample volume: 200 µL
- Elution volume: 50 µL DNA
- Extraction time: 30 minutes /10 samples
- OD: A260/A280 = 1.7 – 2.2
- Storage: Spin columns and buffers can be stored dry at room temperature. Proteinase K stored at 2-8oC).
- Expiration date: 12 months from the date of manufacture
| Component | Storage |
| BL Buffer | Room temperature |
| WB1 Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| Ethanol | |
| 1.5 mL Tube | |
| Silica column | |
| Proteinase K | 2 – 8oC |
DATA EXPERIMENT

Figure 1 The comparison of silica column extraction kit on whole blood samples between ABT (Green) and other foreign suppliers (Red, Blue)

Figure 2: Electrophoresis results of the DNA genome from Whole blood samples by EDTA (1), Citrate (2), Heparin (3), Balance (4)








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