Name: ABT® GRAM STAINING KIT (DD-002) - ABT
Brand: Hóa chất
SKU: DD-002

About ABT ® GRAM STAINING KIT

Intended use: ABT ® Gram Staining Kit is utilized for bacterial staining to differentiate between two bacterial groups, Gram-positive (+) and Gram-negative (-), based on the structural composition of the bacterial cell wall.

Staining capabilities:

Gram-positive bacteria exhibit a violet-green coloration
Gram-negative bacteria display a pink-red hue

Storage: 15 - 30°C, protect from direct sunlight

Expiration date: 12 months from manufacture

Components: The kit includes 4 separate chemical bottles:

Crystal violet solution: Homogeneous liquid slightly viscous liquid, with a purple or violet-green color. Exhibits a distinctive odor.
Lugol solution: Homogeneous, moderately viscous liquid, with a reddish-brown color. Exhibits a distinctive odor.
Decolorizer solution: Homogeneous, transparent, colorless liquid, free from sediment. Exhibits a distinctive odor.
Safranin O solution: Homogeneous, slightly viscous liquid, red in color. Exhibits a distinctive odor.

Packaging: DD-002 (4 bottles x 100 mL/box)

Procedure

Step 1: Evenly distribute the disease specimen or bacteria onto a clean microscope slide.
Step 2: Fix the disease specimen by gently passing it over the flame of an alcohol lamp and allow it to cool.
Step 3: First, apply Crystal Violet solution and let it stand for approximately 30 seconds, then rinse with water.
Step 4: Next, apply Lugol solution to fix the stain for approximately 30 seconds, then rinse with water.
Step 5: Decolor using Decolorizer solution for about 30 seconds, then rinse with water.
Step 6: Finally, apply Safranin O solution for about 30 seconds, then rinse with water.
Step 7: Allow to air-dry.
Step 8: Observe under a microscope.

Note:

Use gloves, goggles, a mask, and a lab coat during the procedure.
Store the product in a dry, cool place, away from direct light, excessive heat, and potential sources of ignition. Close the product tightly after use.
Immediately clean any stains with water to prevent permanent marks on sink surfaces, laboratory bench chairs, or glassware.
Carefully read the instructions for use, pay attention to the expiration date of the product before use. Do not use the product after the expiration date.
Dispose of waste in accordance with legal regulations and national environmental protection guidelines.