Name: ABT® CERVICAL CYTOLOGY KIT (DD-103) - ABT
Brand: Bộ thu và xử lý mẫu
SKU: DD-103

Information about Cervical Cancer and ABT ® Cervical Cytology Kit

Cervical cancer is a disease associated with the abnormal proliferation of epithelial cells in the cervix (the organ connecting the uterus and the vagina), forming tumors in the uterus. Abnormal cervical cells exhibit the following characteristics: multinucleation, hyperchromasia, and cytoplasmic vacuolization around the nucleus. The nuclei of abnormal cells are three times larger than those of normal cells. Changes in nuclear and cytoplasmic morphology form the basis for cytological methods in early screening for cervical cancer. Based on the cell type, cervical cancer is classified into two main types: squamous cell carcinoma (SCC) and adenocarcinoma (AC). SCC occurs in the epithelial cells at the outer cervix and is more common, while AC occurs in the glandular cells of the cervix.

The main cause of cervical cancer is the Human papillomavirus (HPV), a virus that causes papillomas in humans and animals. HPV spreads through genital contact via unsafe sexual activities. According to the World Health Organization (WHO), cervical cancer is the fourth most common cancer among women worldwide. In 2020, there were approximately 604,000 new cases and 342,000 deaths from cervical cancer. About 90% of new cases and deaths occurred in low- and middle-income countries. According to Globocan statistics, 185 out of 204 countries worldwide report on the situation of cervical cancer. Vietnam ranks 91st out of 185 in terms of new incidence rate and 50th out of 185 in terms of mortality rate globally. This shows a significant increase compared to 2018, where the new incidence rate was 99th out of 185, and the mortality rate was 56th out of 185.

ABT ® Cervical Cytology Kit is a collection, processing, and smearing tool for gynecological cells using dual-filter Liquid-Based Cytology (LBC) technology. Cervical smear samples collected at the cervical site are processed to remove mucus and blood in the sample matrix and stabilized in a buffer alcohol solution for up to 1 month at temperatures of 15-25℃.

The ABT ® Cervical Cytology Kit (DD-103) including:

- Sample collection swab:

Swab material: PE+PP
Diameter of the collection head: 19.5±2 mm
Total length: 202±10 mm

– ABT® Cell Preservative Solution:

Homogeneous, clear, colorless liquid, free from impurities and sediment.
Component: Methanol ≥ 35%
Solution pH = 5.5±02

- Double filter cup:

Cup body (upper membrane): PP+PE, 48mm x 27mm (height x diameter)
Cup base (lower membrane): PP+PE, 27mm x 20mm (height x diameter)
Thin smear diameter: 20±2 mm

- Microscope slide:

Slide material: glass
Size: 75mm x 25mm x 1 mm

Manual instruction ABT ® Cervical Cytology Kit

1. Sample preparation:

Cervical smear samples will be collected according to the Ministry of Health's procedure (Appendix 1, Decision 3877/QD-BYT 2019):

Step 1: Insert a speculum into the vagina and adjust its position to ensure a clear view of the cervix.
Step 2: Insert the sampling brush into the cervix, press gently, and rotate the brush clockwise 5 times (Figure 1).
Step 3: Remove the sampling brush from the vagina and place it into the ABT® Cell Preservative Solution vial, gently rotating it 5 to 10 times. The head of the sampling brush is retained by detaching it from the handle, and the handle is disposed of properly.
Step 4: Close the vial and record the patient's information on the label with a pencil.

Store and transport the sample at room temperature (15-25℃) for up to 1 month for cytological examination (liquid-based Pap test) in cervical cancer screening. Additionally, samples collected and preserved using the kit can be processed for HPV-DNA testing.

Figure 1 Position for placing the cervical smear sampling brush.

2. Processing and smearing slides

- Record the patient's information on the frosted end of the electrostatic glass slide with a pencil and on the double-filter cup with a marker.

- Procedure for smearing slides using the dual-filter LBC systems:

Step 1: Install the electrostatic glass slide and the double-filter cup in the appropriate position as specified by the equipment.
Step 2: Vortex the mixture of the smear sample and ABT® Cell Preservative Solution for 15 seconds and pour 1-2 mL of the solution onto the double-filter cup.
Step 3: Select the GYN mode on the system and smear the cells onto the glass slide according to the system's program.

- After smearing, fix the glass slide in 96% ethanol for 15 minutes and stain with PAP according to the procedure of the ABT ® Papanicolaou Staining Kit (DD-083; DD-084).

- Observe and analyze the results under a microscope.

Note:

- Always wear gloves and a mask during the procedure.

– Shake the ABT® Cell Preservative Solution well before opening the cap for use.

– The appropriate sample collection time is within 2 days before or after menstruation.

– Contraindicated in pregnant women.

– Waste must be disposed of according to national environmental protection laws and regulations.

3. Recommendations:

– Some LBC systems used in conjunction with the kit: E-prep 1000 Processor; Huro Path T Sample Slide Processor; Huro Path S Sample Slide Processor; Max-Prep Processor; CellSolutions F50 Cytology Processor; Liquid Based Cytology Processor KCT-06.