Introduction TopSPEC® YHV One-step RT-qPCR Kit
Yellow head shrimp disease caused by Yellow Head Virus (YHV) usually occurs at the time of season change and is concentrated in coastal farming areas with high salinity. The disease usually appears in shrimp over 30-day old. Mortality rate increases gradually, can reach 100% within 7-10 days. When infected, shrimp often have the following symptoms: yellow or brown gills, yellow thorax, pale shells, swollen stomach and intestines.
TopSPEC® YHV One-step RT-qPCR Kit is a test kit to detect the presence of Yellow Head Virus (YHV) causing yellow head disease. Early detection helps to save costs caused by YHV.
TopSPEC® YHV One-step RT-qPCR Kit
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Yellow head virus (YHV) – polyprotein 1ab |
| Sample types | RNA after extraction |
| Input sample volume | 200 µL |
| RNA volume | 5 µL |
| Color channel detection | FAM: detect YHV HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Only YHV is detected |
| Component | YHV One-step RT-qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube. |
| Storage | 12 months at -20oC |
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Read more: Instructional Video for Qualitative Real-time PCR Kit
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