INTRODUCTION
Vibrio parahaemolyticus found in seafood such as shrimp, fish, crab, and shellfish in brackish or marine waters, causing marine cholera – a type of gastrointestinal disease. The habit of consuming raw or undercooked seafood often leads to infection by V. parahaemolyticus. Acute enteritis and mild cholera are the main manifestations of the disease; additionally, the bacterium can enter through wounds and cause systemic sepsis.
TopSPEC® Vibrio parahaemolyticus qPCR Kit is a screening test kit for V. parahaemolyticus bacteria in food microbiology, aiding in food safety control and ensuring health standards.
TopSPEC® Vibrio parahaemolyticus qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Vibrio parahaemolyticus |
| Sample types | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Color channel detection | Texas Red: detect V. parahaemolyticus HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects V. parahaemolyticus |
| Component | V.para qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube. |
| Storage | 12 months at -20oC |
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Read more: Instructional Video for Qualitative Real-time PCR Kit
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