INTRODUCTION
Cholera is a common infectious disease of the digestive tract. Typical symptoms include diarrhea, vomiting, severe dehydration, and exhaustion. The cholera bacterium Vibrio cholera is the direct causative agent, existing in the surrounding environment, as well as in human and animal feces. At various times, cholera has reached pandemic levels and caused severe outbreaks.
TopSPEC® Vibrio cholera qPCR Kit is a screening test kit for V. cholera bacteria in food microbiology, aiding in food safety control and ensuring health standards.
TopSPEC® Vibrio cholera qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Vibrio cholera |
| Sample types | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Color channel detection | FAM: detect V. cholera HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects V. cholera |
| Component | Vibrio cholera qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube. |
| Storage | 12 months at -20oC |
Recommended extraction kit
TopPURE® Crude DNA Extraction Kit
TopPURE® Highpure Viral Extraction Kit
TopPURE® Genomic DNA Extraction Kit
TopPURE® Maga Genomic DNA/RNA Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit
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