Introduction
Vibrio alginolyticus is a Gram-negative bacterium belonging to the Vibrionaceae family, characterized by its motility, facultative anaerobic nature, and typically straight or slightly curved rod-shaped morphology.V. alginolyticus is known to cause red body syndrome in postlarval shrimp. Affected postlarvae exhibit red spots on the base of antennae, head-thorax junction, body, and appendages when infected with Vibrio alginolyticus. Additionally, numerous studies indicate that, along with V. cholera, V. alginolyticus is implicated in causing white feces syndrome in whiteleg shrimp. Therefore, early detection of the presence of this bacterium in aquaculture environments is crucial to mitigate the potential severe consequences it may induce.
TopSPEC® V. alginolyticus qPCR Kit is a testing kit designed to detect the presence of Vibrio alginolyticus in aquatic samples using the Real-time PCR technique. The kit enables the rapid detection of the pathogen with high sensitivity and specificity.
TopSPEC® V. alginolyticus qPCR Kit
Highlights TopSPEC® V.alginolyticus qPCR Kit
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Vibrio alginolyticus – toxR gene |
| Sample types | DNA after extraction |
| DNA volume | 5 µL |
| Color channel detection | FAM: detect Vibrio alginolyticus HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects V. alginolyticus |
| Component | Vibrio alginolyticus qPCR mix, negative control, positive control, internal control, PCR tube |
| Storage | 12 months at -20oC |
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Read more: Instructional Video for Qualitative Real-time PCR Kit









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