INTRODUCTION
Gastroenteritis and dysentery caused by bacilli Shigella contamination in food and drinking water. Shigella causing inflammation of the gastrointestinal mucosa, leading to typical symptoms such as diarrhea, vomiting, fever, and body aches. In addition, the disease can result in complications such as dehydration and electrolyte imbalance, lactose intolerance, and hemolytic syndrome.
TopSPEC® Shigella qPCR Kit is a screening test kit for Shigella bacteria in food microbiology, aiding in food safety control and ensuring health standards.
TopSPEC® Shigella qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Shigella |
| Sample types | DNA after extraction |
| Input sample volume | 200 µL |
| DNA volume | 5 µL |
| Color channel detection | FAM: detect Shigella HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects Shigella |
| Component | Shi qPCR mix, negative control, positive control, internal control (IC), Tube PCR. |
| Storage | 12 months at -20oC |
Recommended extraction kit
TopPURE® Genomic DNA Extraction Kit
TopPURE® Crude DNA Extraction Kit
TopPURE® Maga Genomic DNA/RNA Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit
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