About TopSPEC® nptll Gene qPCR Kit
Neomycin phosphotransferase II (nptll) originated from Escherichia coli Tn5 transposon. NPTII is a type of aminoglycoside phosphotransferase that inactivates aminoglycoside antibiotics, including kanamycin and neomycin. NPTII inactivates kanamycin by catalyzing the transfer of the terminal phosphate group from ATP to kanamycin and is used in plants, as well as in yeast and mammals.
TopSPEC® nptll Gene qPCR Kit is a diagnostic kit designed to detect genetically modified materials by identifying the presence of the Neomycin phosphotransferase II (nptII) gene in plants and various food products using the Real-time PCR technique.
TopSPEC® nptll Gene qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | nptll gene |
| Input sample | DNA after extraction |
| Input sample volume | 5 µL |
| Color channel detection | FAM: detect nptll gene HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects nptll gene |
| Storage | 12 months at -20oC |
| Kit components | nptll gene qPCR, negative control, positive control, internal control (IC), PCR tube |
| Recommended for extraction kit | ||
| Silica column extraction | HE-009 | TopPURE® Plant DNA Extraction Kit |
| HE-013 | TopPURE® Food DNA Extraction Kit | |
Read more: Instructional Video for Qualitative Real-time PCR Kit
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