INTRODUCTION
White tail disease (WTD) or white muscle disease (WMD) occurs in freshwater prawns caused by Macrobrachium Rosenbergii Nodavirus (MrNV) and Extra Small Virus (XSV). Although MrNV is the main cause of the disease, XSV plays an equally important role. XSV is the first nodavirus-related satellite recorded in animals, and is limited to gill tissue, heart, ovary, and head muscle. Therefore, the disease can spread from shrimp seeds to larvae and even the early stages of juveniles. The mortality rate reaches 100% within 5-6 days from the onset of the disease.
TopSPEC® MrNV/XSV One-step RT-qPCR Kit is a test kit that detects the presence of Macrobrachium Rosenbergii Nodavirus (MrNV) and Extra Small Virus (XSV) in the culture environment. Early detection of pathogens helps save costs caused by MrNV/XSV.
The TopSPEC® MrNV/XSV One-step RT-qPCR Kit (undivided master mix)
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Macrobrachium rosenbergii nodavirus (MrNV) và Extra Small Virus (XSV) – RNA dependent RNA Polymerase và capsid protein gene |
| Input sample | RNA after extraction |
| Input sample volume | 200 µL |
| RNA volume | 5 µL |
| Quantitative detection | FAM: MrNV, Cy5: XSV HEX: internal control |
| Flourescent channel | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detect MrNV/XSV |
| Storage | 12 months at -20oC |
| Kit components | MX One-step RT- qPCR mix, negative control, positive control (MrNV, XSV), internal control, PCR tube |
Recommended extraction kit
TopPURE® Highpure viral extraction kit (EXS-021)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional video for the qualitative Real-time PCR kit
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