Introducing TopSPEC® MrNV One-step RT-qPCR Kit
White Tail Disease (WTD) or White Muscle Disease (WMD) occurs in giant freshwater prawns and is caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). MrNV is the primary causative agent of the disease, while XSV also plays a significant role. MrNV infection does not cause mortality in adult shrimp; however, it can be transmitted from broodstock to larvae and juveniles, leading to death. The mortality rate among larvae and juvenile shrimp reaches 100% within 5–6 days after the onset of the disease. Therefore, early screening and detection of the MrNV virus should be prioritized to minimize losses.
TopSPEC® MrNV One-step RT-qPCR Kit is a test kit that detects the presence of Macrobrachium rosenbergii nodavirus (MrNV) in the culture environment. Early detection of pathogens helps save costs caused by MrNV.
The TopSPEC® MrNV One-step RT-qPCR Kit (undivided master mix)
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Macrobrachium Rosenbergii Nodavirus (MrNV) – RNA dependent RNA Polymerase |
| Sample types | RNA after extraction |
| Input sample volume | 200 µL |
| RNA volume | 5 µL |
| Color channel detection | FAM: detect MrNV HEX: internal control |
| Technology | TaqMan probe/One-step RT-qPCR |
| PCR time | 1 hour 45 minutes |
| Specificity | Only MrNV is detected |
| Component | MrNV One-step RT-qPCR mix, Negative control, Positive control, Internal control (IC), PCR tube. |
| Storage | 12 months at -20oC |
Recommended extraction kit
TopPURE® Highpure Viral Extraction Kit (EXS-021)
TopPURE® Maga Genomic DNA/RNA Extraction Kit (HI-612)
Read more: Instructional video for the qualitative Real-time PCR kit
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