Introduction TopSPEC® JEV RT-qPCR Kit
Japanese Encephalitis (JE) is not only a disease affecting humans but also occurs in various mammalian species, particularly livestock such as pigs, cattle, and buffaloes. Following viral entry into the body, it infiltrates the bloodstream and migrates to various organs. Ultimately, during the developmental stage, the JEV causes damage to the liver, kidneys, spleen, muscles, and, ultimately, the central nervous system. Infected hosts typically exhibit compromised immune systems. In particular, pregnant individuals infected with the virus may experience a significantly high rate of miscarriages. The disease is transmissible at any age, highlighting the importance of early detection as the most effective preventive measure for livestock breeders.
TopSPEC® JEV RT-qPCR Kit is a diagnostic assay designed to detect the presence of Japanese encephalitis virus (JEV) in pigs, cattle, horses, buffaloes, and other livestock using One-step RT-qPCR technology targeting the NS2A gene. Early identification of the pathogen helps reduce economic losses associated with JEV infection.

TopSPEC® JEV RT-qPCR Kit
Highlights
- Simple procedure
- PCR time was optimized for 2 hours
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Japanese encephalitis (JEV) – NS2A gene |
| Sample types | Serum sample and whole blood sample |
| Input sample volume | 200 µL |
| RNA volume | 5 µL |
| Color channel detection | FAM: NS2A gene of JEV HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Specificity | Kit only detects JEV |
| Component | JEV RT-qPCR Mix, Negative control, Positive control, Internal control (IC), PCR tube |
| Storage | 12 months at -20oC |
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TopPURE® Viral DNA/RNA Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit








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