Introduction TopSPEC® E.coli O157 qPCR Kit
Escherichia coli O157 is a Gram-negative, aerobic, rod-shaped bacterium with a cell wall structure containing a thin layer of peptidoglycan. The bacteria have pili that enable them to adhere to surfaces, particularly the intestinal mucosa. They also possess an outer membrane containing lipopolysaccharides, which play a crucial role in toxicity. E. coli O157 is recognized as a major cause of severe gastrointestinal illnesses in humans, such as bloody diarrhea and hemolytic uremic syndrome (HUS). It is commonly transmitted through contact or consumption of contaminated food, particularly undercooked beef, untreated water, and unpasteurized milk.
TopSPEC® E.coli O157 qPCR Kit is a screening kit used to detect the presence of Escherichia coli O157 trên mẫu thực phẩm bằng kỹ thuật qPCR dựa trên sự hiện diện của gen rfbE.

TopSPEC® E.coli O157 qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets | Escherichia coli O157 |
| Sample types | DNA after extraction |
| DNA volume | 5 µL |
| Color channel detection | FAM: detect Escherichia coli O157 HEX: internal control (IC) |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Kit only detects Escherichia coli O157 |
| Component | E.coli O157 qPCR mix, negative control, positive control, internal control, PCR tube |
| Storage | 12 months at -20oC |
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Read more: Instructional Video for Qualitative Real-time PCR Kit








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