TopSPEC® E.coli O157 qPCR KIT (SQB-111)

Intended uses: Check for the presence of Escherichia coli O157 (E.coli O157)  in food samples by real-time PCR

Storage: -20oC

Package: 50 tests

Expiration date: 12 months from the date of manufacture

Manufacturer: ABT (Vietnam)

PRODUCT CATEGORY

Introduction TopSPEC® E.coli O157 qPCR Kit

 

Escherichia coli O157 is a Gram-negative, aerobic, rod-shaped bacterium with a cell wall structure containing a thin layer of peptidoglycan. The bacteria have pili that enable them to adhere to surfaces, particularly the intestinal mucosa. They also possess an outer membrane containing lipopolysaccharides, which play a crucial role in toxicity. E. coli O157 is recognized as a major cause of severe gastrointestinal illnesses in humans, such as bloody diarrhea and hemolytic uremic syndrome (HUS). It is commonly transmitted through contact or consumption of contaminated food, particularly undercooked beef, untreated water, and unpasteurized milk.

TopSPEC® E.coli O157 qPCR Kit is a screening kit used to detect the presence of Escherichia coli O157 trên mẫu thực phẩm bằng kỹ thuật qPCR dựa trên sự hiện diện của gen rfbE.

TopSPEC® E.coli O157 qPCR Kit

TopSPEC® E.coli O157 qPCR Kit

Highlights

  • Simple procedure
  • Optimize the PCR time to 1.5 hours.
  • Various input sample types
  • High specificity and sensitivity

Specification

 

TargetsEscherichia coli O157
Sample typesDNA after extraction
DNA volume5 µL
Color channel detection

FAM: detect Escherichia coli O157

HEX: internal control (IC)

Technology TaqMan probe
PCR time1.5h
SpecificityKit only detects Escherichia coli O157
ComponentE.coli O157 qPCR mix, negative control, positive control, internal control, PCR tube
Storage12 months at -20oC

 

Recommended extraction kit

TopPURE® Genomic DNA Extraction Kit 

TopPURE® Food DNA Extraction Kit 

TopPURE® Maga Genomic DNA/RNA Extraction Kit 

Read more: Instructional Video for Qualitative Real-time PCR Kit

SQB-111

50 tests

Manual instruction

Vietnamese version 

English version 

Video

Catalog

Vietnamese version 

English version 

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Công ty TNHH giải pháp y sinh ABT

ABT BIOLOGICAL SOLUTIONS COMPANY LIMITED

ABT is a pioneering biotechnology company specializing in advanced diagnostic solutions that support disease prevention and safeguard public health. With a global vision, ABT continuously invests in research and development to enhance product quality and affirm the scientific standing of Vietnam on the international stage.

Contact us

Factory 6.07 and 5.02, Lot L2, Long Hau – Hiep Phuoc Road, Long Hau Industrial Park, Can Giuoc Commune, Tay Ninh Province, Vietnam

No. 1 and No. 3, 13B Street, An Lac Ward, Ho Chi Minh City, Vietnam

0903 307 258
028 2216 0885

oversea@abtvn.com

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