TopSAFE® AGAROSE ELECTROPHORESIS

Specifications

TopSAFE® Agarose Electrophoresis là bộ kit được thiết kế phục vụ cho việc điện di nucleic acid trên giá thể gel agarose. Bộ kit cung cấp trọn vẹn các hóa chất cần thiết cho việc điện di.

TopSAFE® Agarose Electrophoresis

Manual instruction

- Prepare 1.5% agarose gel: Dissolve 1.5 g agarose in 100 mL of 1X TAE buffer

- Gel casting

• Insert the comb into the casting tray.
• Once agarose is fully dissolved and cooled to 60–70°C, use a 1 mL pipette to apply a thin layer along the dam edge to prevent leakage.
• Slowly pour the remaining agarose to avoid bubbles. Allow the gel to solidify completely.
• Carefully remove the comb after gel solidification

- Load samples at a ratio of 5 μL PCR product + 1 μL 6X GelRed Loading Buffer per well.

- Use the DNA ladder at a ratio of 5 μL DNA ladder and 1 μL 6X GelRed Loading Buffer.

- Electrophoresis at 100V for 40 minutes or until the yellow indicator line reaches the end of the gel.

Note:

• Store reagents at recommended temperatures.
• Do not use expired reagents.
• Use distilled water to dilute 50X TAE buffer to 1X before use
• For optimal results, 1X TAE buffer can be reused up to 5 times if prepared with distilled water.
• Ensure the gel is fully solidified before starting electrophoresis
• Thaw and mix the 100 bp DNA ladder and 6X GelRed Loading Buffer thoroughly before use.
• Use appropriate voltage and duration for each electrophoresis chamber.
• Ensure correct electrode placement during electrophoresis.
• Regularly clean and UV-sterilize the electrophoresis room.

Read more:

• Other Bio-reagents
• Manual instruction