Introduction
– Bacteria Vibrio parahaemolyticus, also known as cholera, is a type of Bacteria in the same group as the bacteria that cause cholera, the bacteria are rod-shaped, slightly curved like a comma and short, are gram-negative bacteria, do not produce spores, and are mobile thanks to a hair at one end.
– They grow well in alkaline and salty environments, found in sea water and marine animals such as shrimp, fish, snails, oysters, etc.
– Vibrio parahaemolyticus is one of the causes of food poisoning. People get sick mainly from eating seafood that is undercooked or contaminated during processing and preservation.
– Common symptoms: Diarrhea and vomiting; There are mild symptoms such as loose stools, watery stools, stools with a slightly fishy smell, green or yellow-green color; Excessive vomiting, diarrhea, and watery stools lead to acute dehydration. Almost no fever, no stomach ache.
– Signs usually last about 3 days, can be mild or severe depending on the case. The disease causes more severe infections in people with reduced resistance.
– Because the disease has symptoms quite similar to enteritis caused by other microbial agents, to diagnose the disease, it is necessary to conduct tests: The patient's stool, vomit or foods suspected of being infected.
– Testing method:
+ Examination under a microscope shows that the bacteria move very quickly.
+ Culturing patient samples to isolate bacteria and prepare antibiograms. Vibrio parahaemolyticus, when isolated, will give a very clear hemolytic reaction on blood agar medium.
Culturing and detecting Vibrio parahaemolyticus requires four consecutive stages, so nucleic acid testing is also used as a method to detect sea cholera to save time in returning results.
TopSENSI® Vibrio parahaemolyticus qPCR Kit is used to detect the presence of Vibrio parahaemolyticus The test is performed using Real-time PCR technology on DNA extracted from various human clinical specimens. The results obtained from this diagnostic kit are intended for clinical reference only and should not be used as the sole basis for diagnosis or exclusion of disease.
Advantage of using the kit detects Vibrio parahaemolyticus
- Simple procedure
- PCR time was optimized for 1 hour 15 minutes
- Various input sample types
- High specificity and sensitivity
Specification
| Extracted sample volume | 200µL and 200mg |
| Input sample volume for PCR | 10µL |
| Sample types | Stool sample/rectal swab sample |
| Analytical sensitivity | 25 copies/reaction |
| Analytical specificity | Kit only detects Vibrio parahaemolyticus |
| Technology | TaqMan Probe |
| Storage | -20°C |
| Fluorescent channels | TexasRed: Vibrio parahaemolyticus HEX: Internal control |
| PCR time | 1 hour 15 minutes |
| Expiration Date | 12 months from date of manufacture |
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