Introduction TopSENSI ® Urease Test Kit
Helicobacter pylori is a spiral-shaped gram-negative bacteria that live in the human stomach. This bacterium is found in the mucous layer above the gastric epithelium, and it does not appear to invade the tissue. However, the lining beneath the H. pylori area is frequently inflamed, a condition known as chronic gastritis. If not diagnosed and detected in time, the disease can develop into gastritis, peptic ulcers, and stomach cancer, one of the most dangerous diseases affecting the digestive system. Currently, people infected with H. pylori, if not detected and treated early, will have the disease for life, so accurate screening and quantification will help treat the disease better.
TopSENSI ® Urease Test Kit is designed to detect Helicobacter pylori from biopsy samples of the stomach's cardiac, fundic, or antral regions. The kit is based on fermentation of H. pylori , which hydrolyzes urea into ammonia, raising the pH of the environment and changing the color of the test sample. However, the test results are for clinical reference only and cannot be used as the sole basis for diagnosis or exclusion of the disease.
Highlights
- Simple procedure
- Fast extraction time
Specification
| Targets | Screening H. pylori |
| Sample types | Stomach biopsy sample |
| Storage | 2 – 8oC, 06 months from the date of manufacture |
| Detection time | 2 – 30 phút |
| Kit components | Urease tube with agar |
Result analysis
| Reagent color | Results | Note |
| Color changes from yellow to dark pink within 5 minutes
| Positive | The sample containing H. pylori exhibits high enzymatic activity. |
| Changes from yellow to pink red within 30 minutes.
| Samples containing low levels of H. pylori or obtained from patients with regressed H. pylori infection. | |
| Changes from yellow to orange after 30 minutes.
| Negative | Color change may result from samples containing very low levels of H. pylori, contamination with Proteus or Morganella species, or temporary effects caused by gastric pH. |
| The medium does not change its yellow color after 30 minutes.
| Negative |
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Read more: Instructional Video for Qualitative Real-time PCR Kit
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