Introduction to TopSENSI® Toxoplasma gondii qPCR Kit
Toxoplasma gondii (T. gondii) is an obligate intracellular parasite capable of infecting various animal species, including humans. It is estimated that about one-third of the global population has been exposed to T. gondii at least once.
Typically, this parasite does not pose a health risk to humans. However, for individuals with weakened immune systems and pregnant women, T. gondii can cause severe complications, affecting the nervous system, muscles, eyes, and heart. It may lead to miscarriage, stillbirth, or congenital conditions such as hydrocephalus and epilepsy in newborns.
When a mother is infected with T. gondii, the risk of transmission to the fetus is high—around 30%, even if the mother shows no symptoms. The transmission rate varies by pregnancy stage: 15% in the first trimester, 30% in the second trimester, and 60% in the third trimester. The severity of the infection for the fetus depends on the timing of maternal exposure, with the greatest risk occurring in the first trimester.

Toxoplasma gondii
TopSENSI ® Toxoplasma gondii qPCR Kit is a diagnostic kit based on Real-time PCR technology, enabling the detection of Toxoplasma gondii extracted from various human sample types. The test results from this kit are intended for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.
Advantages of using the kit detects Toxoplasma gondii
- Simple procedure
- PCR time was optimized for 1 hour 15 minutes
- Various input sample types
- High specificity and sensitivity
Specification
| Extracted sample volume | 200µL |
| Input sample volume for PCR | 10µL |
| Sample types | Blood, cerebrospinal fluid, amniotic fluid, chorionic villi,... |
| Analytical sensitivity | 10 copies/reaction |
| Analytical specificity | Kit only detects Toxoplasma gondii |
| Technology | TaqMan Probe |
| Storage | -20°C |
| Fluorescent channels | FAM: B1 gene of Toxoplasma gondii HEX: Internal control (IC) |
| PCR time | 1 hour 15 minutes |
| Expiration Date | 12 months from date of manufacture |
Recommended extraction kit
TopPURE® Blood DNA Extraction Kit (HI-132)
TopPURE ® Genomic DNA Extraction Kit (HI-112)








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